Article
Assessment of High Throughput Sequencing Quality of Host DNA Enriched From Faeces: A Case From Captive Tiger.
Molecular ecology resources - 1 Apr 2026
Yang Jincheng, Zhang Le, Cui Liangyu, Xu Haitao, Ma Yue, Liu Lulu, Yu Mengjia, Mtui Elizabeth Kamili, Ren Xiaotong, Chu Jianing, Zou Ao, Liu Dan, Zhang Lifeng, Li Huixin, Xu Yanchun, Yang Shuhui
Abstract excerpt
Faeces serve as a widely used non-invasive material in wildlife studies, from which host DNA can be enriched for high-throughput sequencing. However, the characteristics of faecal DNA sequencing, particularly the relationship between sequencing volume and genotyping accuracy or genomic coverage, have remained unclear. This knowledge gap makes it challenging to pre-estimate the required sequencing depth to ensure...
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