Article
Rapid genotyping of bacterial leaf blight resistant genes of rice using loop-mediated isothermal amplification assay.
Molecular biology reports - 1 Jan 2021
Prasannakumar M K, Parivallal Buela P, Manjunatha Chennappa, Pramesh Devanna, Narayan Karthik S, Venkatesh Gopal, Banakar Sahana N, Mahesh H B, Vemanna Ramu S, Rangaswamy K T
Abstract excerpt
The use of resistant (R) genes is the most effective strategy to manage bacterial leaf blight (BLB) disease of rice. Several attempts were made to incorporate R genes into susceptible rice cultivars using marker-assisted backcross breeding (MABB). However, MABB relies exclusively on PCR for foreground selection of R genes, which requires expensive equipment for thermo-cycling and visualization of results; hence,...
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