Article
Isothermal amplification of genetically modified DNA sequences directly from plant tissues lowers the barriers to high-throughput and field-based genotyping.
Journal of agricultural and food chemistry - 28 Oct 2009
Lee David, La Mura Maurizio, Allnutt Theo, Powell Wayne, Greenland Andy
Abstract excerpt
DNA extractions are a major cost for high-throughput genotyping. The loop-mediated isothermal amplification (LAMP) assay has been used for the detection of two genetically modified (GM) related sequences. The amplification of target DNA sequences from leaf and maize seed tissues prepared with minimum preparative treatment (disruption in water) demonstrates the ability of LAMP to work in conditions normally...
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