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Rapid and sensitive detection of human adenovirus types 3 and 7 using CRISPR-Cas12b coupled with multiple cross displacement amplification

29 Apr 2025

Abstract excerpt

• A novel diagnostic method combines multiple cross displacement amplification and CRISPR-Cas12b for HAdV-3 and HAdV-7 detection. • Engineered PAM site in CP1 primer overcomes natural PAM absence in target gene. • This method offers fast, accuracy, and specific detection for HAdV-3 and HAdV-7 infections. • Clinical validation confirms the high diagnostic accuracy in patient samples. Human adenovirus type 3...

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PMID: 40486182
DOI: 10.1016/j.imj.2025.100181

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