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Protocol for the use of Oredsson universal replacement medium for cell banking and routine culturing of monolayer and suspension cultures

29 Apr 2025

Abstract excerpt

The Oredsson universal replacement (OUR) medium is the formulation of a universal xeno-free medium designed for the cultivation of human normal and cancer cells in 2D and 3D cultures. Here, we present a protocol for the use of OUR medium for routine culturing, cell banking, and medium modification for suspension culture. We describe steps for thawing, sub-culturing, and freezing cells. We then detail procedures...

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PMID: 40310726
DOI: 10.1016/j.xpro.2025.103781

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