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Article

Development and evaluation of a next-generation sequencing methodology for measles virus using Oxford Nanopore Technology.

Journal of clinical microbiology - 12 Mar 2025

Zubach Vanessa, Osahan Gurasis, Kolsun Kurt, Pfeifer Claudia, Severini Alberto, Hiebert Joanne

Abstract excerpt

We report the development of a bench protocol and evaluation of bioinformatics pipelines for the whole genome sequence (WGS) of measles virus (MeV) genotype D8. We established a bench protocol using 1 kb amplicons tiling the MeV WGS. Four different pipeline parameters were assessed based on two basecallers and two quality thresholds: Guppy simplex with Q-score thresholds of 20 and 25 (G20 and G25), and Dorado...

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Identifiers

PMID: 39902875
DOI: 10.1128/jcm.01456-24
PMCID: PMC11898632

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