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Protocol to study toxic, immune, and suicidal phenotypes of killer yeast.

STAR protocols - 21 Mar 2025

Prins Rianne C, Billerbeck Sonja

Abstract excerpt

Killer yeast produce antimicrobial proteins, often together with a self-protective immunity factor. Here, we present a protocol for analyzing the toxic, immune, and suicidal phenotypes in Saccharomyces cerevisiae. We describe steps for assessing toxicity via halo assays, suicidality through spot assays, and immunity using microtiter plate growth assays. We detail procedures for yeast culturing, performing the...

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PMID: 39893641
DOI: 10.1016/j.xpro.2025.103611
PMCID: PMC11835635

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