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Second- and third-degree kinship analysis by NGS-based SNP genotyping and evaluation of 2045-SNP performance on limited or degraded DNA.

Forensic science international - 1 Feb 2025

Asari Masaru, Takahashi Yuta, Namba Ryo, Hoshina Chisato, Mori Kanae, Okuda Katsuhiro, Shimizu Keiko

Abstract excerpt

We developed a novel next-generation sequencing-based single-nucleotide polymorphism (SNP) genotyping method for second- and third-degree kinship analysis, and designed 1144- and 2045-SNP panels using one (Set A) and two sets (Sets A and B) of primers. These SNP loci were analyzed in 120 Japanese individuals, and likelihood ratios (LRs) for kinship discrimination were calculated to evaluate the effect of number...

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PMID: 39708709
DOI: 10.1016/j.forsciint.2024.112346

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