Article
Development of an NGS panel containing 42 autosomal STR loci and the evaluation focusing on secondary kinship analysis.
International journal of legal medicine - 1 Nov 2020
Liu Qingxia, Ma Guanju, Du Qingqing, Lu Chaolong, Fu Lihong, Wang Qian, Fu Guangping, Li Shujin, Cong Bin
Abstract excerpt
High-throughput next-generation sequencing (NGS) is a feasible technique to detect considerably more markers and simultaneously obtain length and sequence information in a single reaction. In this study, we developed an NGS panel including 42 commonly used autosomal short tandem repeats (STRs) and amelogenin on the Illumina MiSeq FGx™. Sequencing accuracy was validated by the consistency of 2800M Control DNA...
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