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Cellular Phenotyping of Peripheral Blood Mononuclear Cells from Marburg Virus-Infected Animals.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2025

O'Donnell Kyle L

Abstract excerpt

Cellular phenotyping of immune cells is a critical component of understanding the immunological impact of Marburg virus on the host. Moreover, it is also important to identify correlates of protection in vaccine or therapeutic preclinical and clinical studies. The protocol below describes the isolation and storage of peripheral blood mononuclear cells, proper thawing techniques, cellular stimulation guidelines,...

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PMID: 39585634
DOI: 10.1007/978-1-0716-4256-6_25

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