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Article

Optimized simple culture protocol for inducing mature myotubes from MYOD1-overexpressed human iPS cells.

Scientific reports - 20 Nov 2024

Wada Eiji, Susumu Nao, Okuzaki Yuya, Hotta Akitsu, Sakurai Hidetoshi, Hayashi Yukiko K

Abstract excerpt

The forced expression system of MYOD1, a master gene for myogenic differentiation, can efficiently and rapidly reproduce muscle differentiation of human induced pluripotent stem cells (hiPSCs). Despite these advantages of the MYOD1 overexpression system, developed myotubes are relatively immature and do not recapitulate several aspects of striated muscle fibers. Here, we developed a simple optimized protocol...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 39567611
DOI: 10.1038/s41598-024-79745-w
PMCID: PMC11579357

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