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Article

Bio-orthogonal tuning of matrix properties during 3D cell culture to induce morphological and phenotypic changes.

Nature protocols - 1 Mar 2025

Gao Hanyuan, Pol Mugdha, Makara Colette A, Song Jiyeon, Zhang He, Zou Xiaoyu, Benson Jamie M, Burris David L, Fox Joseph M, Jia Xinqiao

Abstract excerpt

Described herein is a protocol for producing a synthetic extracellular matrix that can be modified in situ during three-dimensional cell culture. The hydrogel platform is established using modular building blocks employing bio-orthogonal tetrazine (Tz) ligation with slow (norbornene, Nb) and fast (trans-cyclooctene, TCO) dienophiles. A cell-laden gel construct is created via the slow, off-stoichiometric Tz/Nb...

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Identifiers

PMID: 39501109
DOI: 10.1038/s41596-024-01066-z
PMCID: PMC11898115

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