Loop-Mediated Isothermal Amplification Detection of Phytophthora kernoviae , P . ramorum , and the P . ramorum NA1 Lineage on a Microfluidic Chip and Smartphone Platform

Abstract excerpt

Rapid, field-deployable assays such as loop-mediated isothermal amplification (LAMP) are critical for detecting nursery and forest pathogens such as Phytophthora ramorum and P. kernoviae to prevent pathogen spread. We developed and validated four LAMP assays for genus-level detection of Phytophthora spp., species-level detection of P. kernoviae and P. ramorum, and lineage-level detection of the P. ramorum NA1...

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