Culture and amplification-free nanopore sequencing for rapid detection of pathogens and antimicrobial resistance genes from urine

Abstract excerpt

Abstract Purpose Urinary Tract Infections (UTIs) are among the most prevalent infections globally. Every year, approximately 150 million people are diagnosed with UTIs worldwide. The current state-of-the-art diagnostic methods are culture-based and have a turnaround time of 2–4 days for pathogen identification and susceptibility testing. Methods This study first establishes an optical density culture-based method...

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