Article
Molecular detection of ceftriaxone resistance in Neisseria gonorrhoeae clinical specimens: a tool for public health control.
Sexually transmitted infections - 17 Oct 2024
Day Michaela Joanne, Boampong Dolcibella, Pitt Rachel, Bari Aisha, Rebec Monica, Saunders John, Fifer Helen, Mbisa Jean Lutamyo, Cole Michelle Jayne
Abstract excerpt
OBJECTIVES: This study aimed to validate and implement a rapid screening assay for molecular detection of the penA-60 allele that is associated with ceftriaxone resistance in Neisseria gonorrhoeae for use on both isolate lysates and clinical specimen DNA extracts. METHODS: A N. gonorrhoeae penA real-time (RT)-PCR was adapted to include a species-specific pap confirmation target and a commercially available...
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