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A high‐fidelity, dual site‐specific integration system in CHO cells by a Bxb1 recombinase

1 Feb 2024

Abstract excerpt

Site-specific integration (SSI) via recombinase mediated cassette exchange (RMCE) has shown advantages over random integration methods for expression of biotherapeutics. As an extension of our previous work developing SSI host cells, we developed a dual-site SSI system having two independent integration sites at different genomic loci, each containing a unique landing pad (LP). This system was leveraged to...

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PMID: 38375559
DOI: 10.1002/biot.202300410

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