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Application of SNaPshot Technology in Semen-Specific cSNP Genetic Marker.

Fa yi xue za zhi - 25 Oct 2023

Tao Rui-Yang, Wang Shou-Yu, Yuan Chun-Yan, Xia Ruo-Cheng, Li Cheng-Tao

Abstract excerpt

OBJECTIVES: To explore the feasibility of genetic marker detection of semen-specific coding region single nucleotide polymorphism (cSNP) based on SNaPshot technology in semen stains and mixed body fluid identification. METHODS: Genomic DNA (gDNA) and total RNA were extracted from 16 semen stains and 11 mixtures composed of semen and venous blood, and the total RNA was reverse transcribed into complementary DNA...

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PMID: 38006266
DOI: 10.12116/j.issn.1004-5619.2021.510804

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