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Portable, single nucleotide polymorphism-specific duplex assay for virus surveillance in wastewater.

The Science of the total environment - 20 Feb 2024

Oh Chamteut, Xun Guanhua, Lane Stephan Thomas, Petrov Vassily Andrew, Zhao Huimin, Nguyen Thanh H

Abstract excerpt

The Argonaute protein from the archaeon Pyrococcus furiosus (PfAgo) is a DNA-guided nuclease that targets DNA with any sequence. We designed a virus detection assay in which the PfAgo enzyme cleaves the reporter probe, thus generating fluorescent signals when amplicons from a reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay contain target sequences. We confirmed that the RT-LAMP-PfAgo...

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PMID: 37992833
DOI: 10.1016/j.scitotenv.2023.168701

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