Article
Embryonic microinjection of ribonucleoprotein complex (Cas9+sgRNA) of white gene in melon fly, Zeugodacus cucurbitae (Coquillett) (Diptera: Tephritidae) produced white eye phenotype.
Archives of insect biochemistry and physiology - 1 Dec 2023
Pradhan Sanjay Kumar, Karuppannasamy Ashok, Sujatha Parvathy Madhusoodanan, Nagaraja Bhargava Chikmagalur, Narayanappa Anu Cholenahalli, Chalapathi Pradeep, Dhawane Yogi, Bynakal Shivanna, Riegler Markus, Maligeppagol Manamohan, Ramasamy Asokan
Abstract excerpt
Melon fly, Zeugodacus cucurbitae (Coquillett) is a major pest of cucurbitaceous crops, and causes substantial yield losses and economic costs. CRISPR/Cas9 is a rapid and effective site-specific genome editing tool for the generation of genetic changes that are stable and heritable. The CRISPR/Cas9 tool uses synthetically designed single guide RNA (sgRNA) that is complementary to the target gene and guides the...
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