Article
Deletions within intronic T-DNA lead to reversion of T-DNA mutant phenotypes.
The Plant journal : for cell and molecular biology - 1 Jan 2024
Thulasi Devendrakumar Karen, Goldstein Madeleine, Kronstad James, Li Xin
Abstract excerpt
Agrobacterium-mediated transformation enables random transfer-DNA (T-DNA) insertion into plant genomes. T-DNA insertion into a gene's exons, introns or untranscribed regions close to the start or stop codon can disrupt gene function. Such T-DNA mutants have been useful for reverse genetics analysis, especially in Arabidopsis thaliana. As T-DNAs are inserted into genomic DNA, they are generally believed to be...
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