Article
Plasmodium falciparum population structure inferred by msp1 amplicon sequencing of parasites collected from febrile patients in Kenya.
Malaria journal - 9 Sept 2023
Andika Brian, Mobegi Victor, Gathii Kimita, Nyataya Josphat, Maina Naomi, Awinda George, Mutai Beth, Waitumbi John
Abstract excerpt
BACKGROUND: Multiplicity of infection (MOI) is an important measure of Plasmodium falciparum diversity, usually derived from the highly polymorphic genes, such as msp1, msp2 and glurp as well as microsatellites. Conventional methods of deriving MOI lack fine resolution needed to discriminate minor clones. This study used amplicon sequencing (AmpliSeq) of P. falciparum msp1 (Pfmsp1) to measure spatial and...
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