Article
Activity- and gene-based quantification of enteric viruses, F- specific RNA phage genogroups, pepper mild mottle virus, and Escherichia coli in surface water.
The Science of the total environment - 15 Dec 2023
Hata Akihiko, Meuchi Yuno, Liu Miaomiao, Torii Shotaro, Katayama Hiroyuki
Abstract excerpt
Polymerase chain reaction (PCR) is widely applied for the monitoring of pathogenic viruses in water environments. To date, several pretreatments to selectively detect genes from infectious viruses via PCR have been developed. This study was aimed to characterize and validate methods for quantifying active viruses and indicators and to evaluate the proportion of their active fractions in surface water (n = 42)....
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