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Digital PCR-based assessment of pathogens in wastewater and antibiotic resistance genes in drinking water of the Kathmandu Valley, Nepal

12 Jun 2025

Abstract excerpt

Digital polymerase chain reaction (dPCR) is a significant advancement in health-related water microbiology, enabling absolute quantification without standard curves. By partitioning samples into thousands of individual reactions, dPCR allows for precise quantification even in the presence of inhibitory substances common in environmental samples. This study evaluated the applicability of dPCR to detect...

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PMID: 40513271
DOI: 10.1016/j.jenvman.2025.126120

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