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Article

Protocol for measuring BRAF autoinhibition in live cells using a proximity-based NanoBRET assay.

STAR protocols - 15 Sept 2023

Spencer-Smith Russell, Morrison Deborah K

Abstract excerpt

BRAF is frequently activated via mutation in human cancer and the RASopathy syndromes; however, for BRAF activation to occur, autoinhibitory interactions between the regulatory and catalytic domains must be relieved. Here, we present a proximity-based NanoBRET (bioluminescence resonance energy transfer) assay for real-time measurement of BRAF autoinhibition in live cells. We describe steps for seeding,...

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Identifiers

PMID: 37590148
DOI: 10.1016/j.xpro.2023.102461
PMCID: PMC10440347

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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