Article
A highly sensitive PCR method for A1 allele detection in A2 milk samples without DNA isolation.
Animal science journal = Nihon chikusan Gakkaiho - 1 Jan 2000
Watanabe Ayumi, Munakata Kyo, Muto Miyabi, Kuramoto Takashi
Abstract excerpt
We previously developed a genotyping method to detect the A1 and A2 alleles of the bovine β-casein gene. This method required DNA extraction from hair samples. Recently, demand for A2 milk (milk from cows homozygous for the A2 allele) has increased, and dairy farms are required to have certification to produce A2 milk. Here, we describe the development of a new, simple, and sensitive genotyping method for the...
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