Article
New high-sensitive rhAmp method for A1 allele detection in A2 milk samples.
Food chemistry - 30 May 2020
Giglioti Rodrigo, Gutmanis Gunta, Katiki Luciana Morita, Okino Cintia Hiromi, de Sena Oliveira Márcia Cristina, Vercesi Filho Anibal Eugênio
Abstract excerpt
Cows' milk may contain two types of β-casein: A1 and A2. A1 digestion is associated with the release of β-casomorphine-7 peptide, which can cause adverse gastrointestinal effects. Two methods - high-resolution melting (HRM) and rhAmp® SNP genotyping - were developed to identify the β-casein gene (CSN2) A1 and A2 alleles directly in milk. DNA milk samples from 45 animals were examined and 10 samples were also...
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