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Detecting the Neuraminidase R294K Mutation in Avian Influenza A (H7N9) Virus Using Reverse Transcription Droplet Digital PCR Method.

Viruses - 17 Apr 2023

Lou Xiuyu, Yan Hao, Su Lingxuan, Sun Yi, Wang Xinyin, Gong Liming, Chen Yin, Li Zhen, Fang Zhongbiao, Mao Haiyan, Chen Keda, Zhang Yanjun

Abstract excerpt

The R294K mutation in neuraminidase (NA) causes resistance to oseltamivir in the avian influenza virus H7N9. Reverse transcription droplet digital polymerase chain reaction (RT-dd PCR) is a novel technique for detecting single-nucleotide polymorphisms. This study aimed to develop an RT-dd PCR method for detecting the R294K mutation in H7N9. Primers and dual probes were designed using the H7N9 NA gene and the...

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Identifiers

PMID: 37112963
DOI: 10.3390/v15040983
PMCID: PMC10146270

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