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Development and validation of a droplet digital PCR assay for Nipah virus quantitation

28 Sept 2024

Abstract excerpt

BACKGROUND: Nipah virus (NiV) is a zoonotic pathogen that poses a significant threat because of its wide host range, multiple transmission modes, high transmissibility, and high mortality rates, affecting both human health and animal husbandry. In this study, we developed a one-step reverse transcription droplet digital PCR (RT-ddPCR) assay that targets the N gene of NiV. RESULTS: Our RT-ddPCR assay exhibited...

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PMID: 39342301
DOI: 10.1186/s12917-024-04245-y

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