BLEACH&STAIN 15-marker Multiplexed Imaging in 3,098 Human Carcinomas Reveals Six Major PD-L1-driven Immune Phenotypes with Distinct Spatial Orchestration.

doi:10.1158/1541-7786.MCR-22-0593

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Community

Back to search

Article

BLEACH&STAIN 15-marker Multiplexed Imaging in 3,098 Human Carcinomas Reveals Six Major PD-L1-driven Immune Phenotypes with Distinct Spatial Orchestration.

Molecular cancer research : MCR - 1 Jun 2023

Bady Elena, Möller Katharina, Mandelkow Tim, Raedler Jonas B, Yang Cheng, Ebner Julia, Lurati Magalie C J, Simon Ronald, Vettorazzi Eik, Büscheck Franziska, Luebke Andreas M, Dum David, Menz Anne, Sauter Guido, Höflmayer Doris, Weidemann Sören, Fraune Christoph, Uhlig Ria, Bernreuther Christian, Jacobsen Frank, Clauditz Till S, Wilczak Waldemar, Burandt Eike, Steurer Stefan, Minner Sarah, Lennartz Maximilian, Blessin Niclas C

Abstract excerpt

Multiplex fluorescence IHC (mfIHC) approaches were yet either limited to six markers or limited to a small tissue size that hampers translational studies on large tissue microarray cohorts. Here we have developed a BLEACH&STAIN mfIHC method that enabled the simultaneous analysis of 15 biomarkers (PD-L1, PD-1, CTLA-4, panCK, CD68, CD163, CD11c, iNOS, CD3, CD8, CD4, FOXP3, CD20, Ki67, and CD31) in 3,098 tumor...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Artificial Intelligence

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to B7-H1 Antigen

Share to Carcinoma

Share to Coloring Agents

Share to Forkhead Transcription Factors

Share to Lymphocytes, Tumor-Infiltrating

Share to Programmed Cell Death 1 Receptor

Share to Tumor Microenvironment

Source

Open in PubMed Open full text

Identifiers

PMID: 36976297
DOI: 10.1158/1541-7786.MCR-22-0593
PMCID: PMC10233353

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

BLEACH&STAIN 15-marker Multiplexed Imaging in 3,098 Human Carcinomas Reveals Six Major PD-L1-driven Immune Phenotypes with Distinct Spatial Orchestration.PMID 36976297Read ↗

Multiplex Immunohistochemistry and High-Throughput Image Analysis for Evaluation of Spatial Tumor Immune Cell Markers in Human Breast Cancer: Preliminary Results from the Nashville Breast Health StudySemantic proximity Read ↗

A Single-Cell Tumor Immune Atlas for Precision OncologySemantic proximity Read ↗

Automated tumor immunophenotyping predicts clinical benefit from anti-PD-L1 immunotherapySemantic proximity Read ↗

Image-based multiplex immune profiling of cancer tissues: translational implications. A report of the International Immuno-oncology Biomarker Working Group on Breast Cancer.Semantic proximity Read ↗

Delineating spatial cell-cell interactions in the solid tumour microenvironment through the lens of highly multiplexed imagingSemantic proximity Read ↗

Single Cell Spatial Analysis and Biomarker Discovery in Hodgkin LymphomaSemantic proximity Read ↗

ImmuNet: A Segmentation-Free Machine Learning Pipeline for Immune Landscape Phenotyping in Tumors by Muliplex ImagingSemantic proximity Read ↗

Tumor-agnostic transcriptome-based classifier identifies spatial infiltration patterns of CD8+T cells in the tumor microenvironment and predicts clinical outcome in early-phase and late-phase clinical trialsSemantic proximity Read ↗

Integrative Spatial Analysis of H&E and IHC Images Identifies Prognostic Immune Subtypes Correlated with Progression-Free Survival in Human Papillomavirus (HPV)-Related Oropharyngeal Squamous Cell CarcinomaSemantic proximity Read ↗

A comprehensive single-cell map of T cell exhaustion-associated immune environments in human breast cancer.Semantic proximity Read ↗

Spatial immunophenotypes predict response to anti-PD1 treatment and capture distinct paths of T cell evasion in triple negative breast cancerSemantic proximity Read ↗

Pan-cancer prediction of tumor immune activation and response to immune checkpoint blockade from tumor transcriptomics and histopathologySemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.