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Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers

10 Feb 2023

Abstract excerpt

Intestinal organoids are increasingly being used to study the gut epithelium for digestive disease modeling, or to investigate interactions with drugs, nutrients, metabolites, pathogens, and the microbiota. Methods to culture intestinal organoids are now available for multiple species, including pigs, which is a species of major interest both as a farm animal and as a translational model for humans, for example,...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 36847381
DOI: 10.3791/64917

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