Article
Amplification refractory mutation system based real-time PCR (ARMS-qPCR) for rapid resistance characterization of Tribolium castaneum to phosphine.
Pesticide biochemistry and physiology - 1 Oct 2022
Wang Kangxu, Che Meiling, Chen Erhu, Jian Fuji, Tang Peian
Abstract excerpt
Resistance of Tribolium castaneum to phosphine is related to point mutations in DNA code corresponding to amino acid changes associated with a core metabolic enzyme dihydrolipoamide dehydrogenase (DLD), but the mutation patterns vary among different resistant populations. Thus, there is a great need to develop a cost-effective method to detect core mutations in T. castaneum, which would be the key factor to...
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