Article
PRPF31 interacts with PRPH2 confirmed by co-immunoprecipitation and co-localization.
Biochemical and biophysical research communications - 12 Nov 2022
Xiao Xiaoqiang, Ling Fangyi, Chen Chong-Bo, Liang Jiajian, Cao Yingjie, Xu Yanxuan, Chen Haoyu
Abstract excerpt
Both PRPF31 and PRPH2 are the causative genes for retinitis pigmentosa. And both of them are associated with the balance of rhodopsin. In this study, we aim to investigate the co-expression and interaction of PRPF31 and PRPH2. We used PRPF31-eGFP, PRPF31-3xFlag and PRPH2-mCherry vectors were transfected into HEK293T and APRE-19 cells. Immunoblotting and co-immunoprecipitation (Co-IP) were used for gene expression...
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