Article
Efficient breeding of low glutelin content rice germplasm by simultaneous editing multiple glutelin genes via CRISPR/Cas9.
Plant science : an international journal of experimental plant biology - 1 Nov 2022
Chen Zhihui, Du Hongxu, Tao Yajun, Xu Yang, Wang Fangquan, Li Bin, Zhu Qian-Hao, Niu Hongbin, Yang Jie
Abstract excerpt
Chronic kidney disease (CKD) and phenylketonuria (PKU) patients need to eat rice with low glutelin content. Therefore, breeding low glutelin content rice varieties with high yield and delicious taste is one of the major goals of rice breeders due to the high demand for the product. In this study, we designed three sgRNAs targeting nine glutelin genes and generated nine T-DNA-free homozygous editing lines with...
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