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Sperm Chromatin Structure Assay (SCSA®) for Fertility Assessment

1 Aug 2022

Abstract excerpt

Abstract The Sperm Chromatin Structure Assay (SCSA ® ) is a federally registered protocol for simultaneous flow cytometric measures of sperm DNA integrity and chromatin structure. Fresh or frozen/thawed raw semen samples are diluted in buffer to a sperm concentration of ∼1–2×10 6 /ml and then treated with a pH 1.20 buffer for 30 s to open the DNA strands at sites of DNA strand breaks. The sperm are then stained...

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PMID: 35926128
DOI: 10.1002/cpz1.508

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