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Mutations of Asn321 and Glu322 Improve Resistance of 4-Hydroxyphenylpyruvate Dioxygenase SpHPPDm to Topramezone.

Journal of agricultural and food chemistry - 10 Aug 2022

Wang Haiyan, Lei Peng, Liu Bin, Zhu Jianchun, He Qin, Chen Le, He Jian

Abstract excerpt

As a highly efficient 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitor herbicide, topramezone is an ideal target for herbicide-resistant genetic engineering. In this study, two mutants, K-19 (N321Y) and K-63 (Q166R/E322V), with topramezone resistance increased by 205.3 and 58.5%, respectively, were screened from the random mutation library of SpHPPDm, a topramezone-resistant HPPD mutant that we previously...

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PMID: 35856450
DOI: 10.1021/acs.jafc.2c02327

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