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Article

Development of a simple genotyping method based on indel mutations to rapidly screen SARS-CoV-2 circulating variants: Delta, Omicron BA.1 and BA.2.

Journal of virological methods - 1 Sept 2022

Ayadi Wajdi, Taktak Awatef, Gargouri Saba, Smaoui Fahmi, Chtourou Amel, Skouri-Gargouri Houda, Derbel Rihab, Sassi Azza Hadj, Gargouri Ali, Hammami Adnene, Karray-Hakim Héla, Mokdad-Gargouri Raja, Fki-Berrajah Lamia

Abstract excerpt

The high need of rapid and flexible tools that facilitate the identification of circulating SARS-CoV-2 Variants of Concern (VOCs) remains crucial for public health system monitoring. Here, we develop allele-specific (AS)-qPCR assays targeting three recurrent indel mutations, ΔEF156-157, Ins214EPE and ΔLPP24-26, in spike (S) gene to identify the Delta VOC and the Omicron sublineages BA.1 and BA.2, respectively....

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Identifiers

PMID: 35724698
DOI: 10.1016/j.jviromet.2022.114570
PMCID: PMC9212420

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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