Article
Novel Multiplex PCR Method and Genome Sequence-Based Analog for High-Resolution Subclonal Assignment and Characterization of Escherichia coli Sequence Type 131 Isolates.
Microbiology spectrum - 29 Jun 2022
Johnston Brian D, Gordon David M, Burn Samantha, Johnson Timothy J, Weber Bonnie P, Miller Elizabeth A, Johnson James R
Abstract excerpt
Escherichia coli sequence type 131 (ST131) is a pandemic, multidrug-resistant extraintestinal pathogen. The multiple distinctive ST131 subclones differ for rfb and fliC alleles (O and H antigens), fimH allele (type-1 fimbriae adhesin), resistance phenotype and genotype, clinical correlates, and host predilection. Current PCR assays for detecting ST131 and its main subclones offer limited sub-ST characterization....
Topics
- Anti-Bacterial Agents
- Escherichia coli
- Escherichia coli Infections
- Escherichia coli Proteins
- Fluoroquinolones
- Genotype
- Humans
- Multiplex Polymerase Chain Reaction
- beta-Lactamases