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Targeted Sanger sequencing to recover key mutations in SARS-CoV-2 variant genome assemblies produced by next-generation sequencing.

Microbial genomics - 1 Mar 2022

Singh Lavanya, San James E, Tegally Houriiyah, Brzoska Pius M, Anyaneji Ugochukwu J, Wilkinson Eduan, Clark Lindsay, Giandhari Jennifer, Pillay Sureshnee, Lessells Richard J, Martin Darren Patrick, Furtado Manohar, Kiran Anmol M, de Oliveira Tulio

Abstract excerpt

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is adaptively evolving to ensure its persistence within human hosts. It is therefore necessary to continuously monitor the emergence and prevalence of novel variants that arise. Importantly, some mutations have been associated with both molecular diagnostic failures and reduced or abrogated next-generation sequencing (NGS) read coverage in some genomic...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 35294336
DOI: 10.1099/mgen.0.000774
PMCID: PMC9176282

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