Article
Analyzing cachectic phenotypes in the muscle and fat body of Drosophila larvae.
STAR protocols - 18 Mar 2022
Dark Callum, Cheung Shane, Cheng Louise Y
Abstract excerpt
Drosophila has become a popular model for examining the metabolic wasting syndrome, cachexia, characterized by degradation of muscles and fat. Here we present a protocol for quick and consistent scoring of muscle detachment, fat body lipid droplet size, and extracellular matrix (ECM) quantifications in Drosophila larvae. We detail the procedures for dissecting, staining, and imaging third instar Drosophila larval...
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