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Article

Method Matters: Effect of Purification Technology on Neutrophil Phenotype and Function.

Frontiers in immunology - 1 Jan 2022

Blanter Marfa, Cambier Seppe, De Bondt Mirre, Vanbrabant Lotte, Pörtner Noëmie, Abouelasrar Salama Sara, Metzemaekers Mieke, Marques Pedro Elias, Struyf Sofie, Proost Paul, Gouwy Mieke

Abstract excerpt

Neutrophils are the most abundant leukocytes in human blood and the first cells responding to infection and injury. Due to their limited ex vivo lifespan and the impossibility to cryopreserve or expand them in vitro, neutrophils need to be purified from fresh blood for immediate use in experiments. Importantly, neutrophil purification methods may artificially modify the phenotype and functional characteristics of...

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Identifiers

PMID: 35222394
DOI: 10.3389/fimmu.2022.820058
PMCID: PMC8866851

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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