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Unraveling novel and rare mutations for alpha-amylase and key transcription factors in EMS-induced wheat mutants for amylose by TILLING.

Molecular biology reports - 1 Jun 2022

Sharma Vinita, Mishra Ankita, Sharma Himanshu, Kumar Pankaj, Roy Joy K

Abstract excerpt

BACKGROUND: TILLING (Targeting Induced Local Lesions in Genomes) is a reverse-genetic strategy that is used to locate an allelic series of induced point mutations in genes of interest. High-throughput TILLING allows the rapid and cost-effective detection of induced point mutations in populations of chemically mutagenized individuals. Grain amylose content is the major constraints for its nutritional quality and...

Topics

Amylose
Mutation
Transcription Factors
Triticum
alpha-Amylases

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 35092561
DOI: 10.1007/s11033-022-07155-0

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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