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Unraveling novel and rare mutations for alpha-amylase and key transcription factors in EMS-induced wheat mutants for amylose by TILLING.

Molecular biology reports - 1 Jun 2022

Sharma Vinita, Mishra Ankita, Sharma Himanshu, Kumar Pankaj, Roy Joy K

Abstract excerpt

BACKGROUND: TILLING (Targeting Induced Local Lesions in Genomes) is a reverse-genetic strategy that is used to locate an allelic series of induced point mutations in genes of interest. High-throughput TILLING allows the rapid and cost-effective detection of induced point mutations in populations of chemically mutagenized individuals. Grain amylose content is the major constraints for its nutritional quality and...

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PMID: 35092561
DOI: 10.1007/s11033-022-07155-0

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