A reverse genetic, nontransgenic approach to wheat crop improvement by TILLING.

doi:10.1038/nbt1043

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Article

A reverse genetic, nontransgenic approach to wheat crop improvement by TILLING.

Nature biotechnology - 1 Jan 2005

Slade Ann J, Fuerstenberg Susan I, Loeffler Dayna, Steine Michael N, Facciotti Daniel

Abstract excerpt

We report the use of TILLING (targeting induced local lesions in genomes), a reverse genetic, nontransgenic method, to improve a quality trait in a polyploid crop plant. Waxy starches, composed mostly of amylopectin, have unique physiochemical properties. Wheat with only one or two functional waxy genes (granule-bound starch synthase I, or GBSSI) produces starch with intermediate levels of amylopectin. We have...

Topics

Alleles
Amylopectin
Amylose
Crops, Agricultural
DNA Mutational Analysis
DNA Primers
Gene Deletion
Gene Library
Genes, Plant
Genome
Genome, Plant
Heterozygote

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Homozygote

Models, Genetic

Mutation

Phenotype

Plants, Genetically Modified

Polymerase Chain Reaction

Source

Open in PubMed

Identifiers

PMID: 15580263
DOI: 10.1038/nbt1043

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