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Article

A reverse genetic, nontransgenic approach to wheat crop improvement by TILLING.

Nature biotechnology - 1 Jan 2005

Slade Ann J, Fuerstenberg Susan I, Loeffler Dayna, Steine Michael N, Facciotti Daniel

Abstract excerpt

We report the use of TILLING (targeting induced local lesions in genomes), a reverse genetic, nontransgenic method, to improve a quality trait in a polyploid crop plant. Waxy starches, composed mostly of amylopectin, have unique physiochemical properties. Wheat with only one or two functional waxy genes (granule-bound starch synthase I, or GBSSI) produces starch with intermediate levels of amylopectin. We have...

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PMID: 15580263
DOI: 10.1038/nbt1043

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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