Article
DAJIN enables multiplex genotyping to simultaneously validate intended and unintended target genome editing outcomes.
PLoS biology - 1 Jan 2022
Kuno Akihiro, Ikeda Yoshihisa, Ayabe Shinya, Kato Kanako, Sakamoto Kotaro, Suzuki Sayaka R, Morimoto Kento, Wakimoto Arata, Mikami Natsuki, Ishida Miyuki, Iki Natsumi, Hamada Yuko, Takemura Megumi, Daitoku Yoko, Tanimoto Yoko, Dinh Tra Thi Huong, Murata Kazuya, Hamada Michito, Muratani Masafumi, Yoshiki Atsushi, Sugiyama Fumihiro, Takahashi Satoru, Mizuno Seiya
Abstract excerpt
Genome editing can introduce designed mutations into a target genomic site. Recent research has revealed that it can also induce various unintended events such as structural variations, small indels, and substitutions at, and in some cases, away from the target site. These rearrangements may result in confounding phenotypes in biomedical research samples and cause a concern in clinical or agricultural...
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