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Robust LC3B lipidation analysis by precisely adjusting autophagic flux

7 Jan 2022

Abstract excerpt

Autophagic flux can be quantified based on the accumulation of lipidated LC3B in the presence of late-stage autophagy inhibitors. This method has been widely applied to identify novel compounds that activate autophagy. Here we scrutinize this approach and show that bafilomycin A1 (BafA) but not chloroquine is suitable for flux quantification due to the stimulating effect of chloroquine on non-canonical...

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PMID: 34996966
DOI: 10.1038/s41598-021-03875-8

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