Article
A versatile inhibitor of digestive enzymes in Aedes aegypti larvae selected from a pacifastin (TiPI) phage display library.
Biochemical and biophysical research communications - 29 Jan 2022
Manzato Veronica Moraes, Torquato Ricardo Jose Soares, Lemos Francisco Jose Alves, Nishiduka Erika, Tashima Alexandre Keiji, Tanaka Aparecida Sadae
Abstract excerpt
In Brazil, the major vector of arboviruses is Aedes aegypti, which can transmit several alpha and flaviviruses. In this work, a pacifastin protease inhibitor library was constructed and used to select mutants for Ae. aegypti larvae digestive enzymes. The library contained a total of 3.25 × 105 cfu with random mutations in the reactive site (P2-P2'). The most successfully selected mutant, TiPI6, a versatile...
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