Article
Development of a multiplex PCR assay for the detection of key genes associated with Pasteurella multocida subspecies.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc - 1 Mar 2022
Ujvári Barbara, Gantelet Hubert, Magyar Tibor
Abstract excerpt
The ability to distinguish among the subspecies of Pasteurella multocida isolates is important epidemiologically; however, classification at the subspecies level based on the results of conventional biochemical tests (fermentation of sorbitol and dulcitol) is reportedly not accurate in all cases. Therefore, we developed a rapid, multiplex PCR assay to differentiate among the 3 subspecies of P. multocida. The PCR...
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