Reverse Genetics with a Full-Length Infectious cDNA Clone of Bovine Torovirus.

doi:10.1128/JVI.01561-21

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Article

Reverse Genetics with a Full-Length Infectious cDNA Clone of Bovine Torovirus.

Journal of virology - 9 Feb 2022

Ujike Makoto, Etoh Yuka, Urushiyama Naoya, Taguchi Fumihiro, Asanuma Hideki, Enjuanes Luis, Kamitani Wataru

Abstract excerpt

Historically part of the coronavirus (CoV) family, torovirus (ToV) was recently classified in the new family Tobaniviridae. While reverse genetics systems have been established for various CoVs, none exist for ToVs. Here, we developed a reverse genetics system using an infectious full-length cDNA clone of bovine ToV (BToV) in a bacterial artificial chromosome (BAC). Recombinant BToV harboring genetic markers had...

Topics

Animals
Cattle
Cattle Diseases
Cell Line
Cells, Cultured
Chromosomes, Artificial, Bacterial
Cloning, Molecular
DNA, Complementary
Genes, Reporter
Genome, Viral
Hemagglutinins, Viral

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Plasmids

Reverse Genetics

Torovirus

Torovirus Infections

Transfection

Source

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Identifiers

PMID: 34817201
DOI: 10.1128/JVI.01561-21
PMCID: PMC8827028

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