Article
Optimization of BSA-seq experiment for QTL mapping.
G3 (Bethesda, Md.) - 4 Jan 2022
Huang Likun, Tang Weiqi, Wu Weiren
Abstract excerpt
Deep sequencing-based bulked segregant analysis (BSA-seq) has become a popular approach for quantitative trait loci (QTL) mapping in recent years. Effective statistical methods for BSA-seq have been developed, but how to design a suitable experiment for BSA-seq remains unclear. In this paper, we show in theory how the major experimental factors (including population size, pool proportion, pool balance, and...
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