Article
Removing the major allergen Bra j I from brown mustard (Brassica juncea) by CRISPR/Cas9.
The Plant journal : for cell and molecular biology - 1 Feb 2022
Assou Juvenal, Zhang Dingbo, Roth Kristian D R, Steinke Stephan, Hust Michael, Reinard Thomas, Winkelmann Traud, Boch Jens
Abstract excerpt
Food allergies are a major health issue worldwide. Modern breeding techniques such as genome editing via CRISPR/Cas9 have the potential to mitigate this by targeting allergens in plants. This study addressed the major allergen Bra j I, a seed storage protein of the 2S albumin class, in the allotetraploid brown mustard (Brassica juncea). Cotyledon explants of an Indian gene bank accession (CR2664) and the German...
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