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Full-Length Transcriptome: A Reliable Alternative for Single-Cell RNA-Seq Analysis in the Spleen of Teleost Without Reference Genome.

Frontiers in immunology - 1 Jan 2021

Huang Lixing, Qiao Ying, Xu Wei, Gong Linfeng, He Rongchao, Qi Weilu, Gao Qiancheng, Cai Hongyan, Grossart Hans-Peter, Yan Qingpi

Abstract excerpt

Fish is considered as a supreme model for clarifying the evolution and regulatory mechanism of vertebrate immunity. However, the knowledge of distinct immune cell populations in fish is still limited, and further development of techniques advancing the identification of fish immune cell populations and their functions are required. Single cell RNA-seq (scRNA-seq) has provided a new approach for effective in-depth...

Topics

Animals
B-Lymphocyte Subsets
Cluster Analysis
Fish Proteins
Gene Expression Profiling
Macrophages
Monocytes
Perciformes
Phenotype
RNA-Seq
Single-Cell Analysis

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Spleen

T-Lymphocyte Subsets

Transcriptome

Source

Open in PubMed Open full text

Identifiers

PMID: 34646272
DOI: 10.3389/fimmu.2021.737332
PMCID: PMC8502891

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

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